Basic Principles
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Minimize all chemical exposures
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Use adequate ventilation
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Take exposure limits seriously
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Never underestimate risk
Be Familiar with
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Chemical container labels and material safety and data sheets
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Permissible exposure limits for chemicals used
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Physical and health hazards of chemicals
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Personal protective equipment
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When hazardous chemical activities need pre-approval
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What to do in an emergency
Use Required Ventilation and Protective Equipment
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Check for proper hood function before each use
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Don't store materials on hoods or block vents / air flow
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Keep hood closed except when making adjustments
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Wear assigned protective clothing in all laboratory settings
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Wear closed-toed shoes that fully cover feet
Store, Handle, Dispose of Chemicals Safely
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Only keep chemicals needed for the current task in the lab
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Regularly check inventory lab chemicals; store/dispose of what's not needed
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Store chemicals in labeled and appropriate containers
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Store highly toxic substances or opened containers in unbreakable secondary containers
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Store chemicals in designated, well-ventilated rooms
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Don't use storerooms for preparation or repackaging
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Check condition of stored chemicals and containers periodically
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Store breakable containers in chemically resistant trays or overwrap containers
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Wrap evacuated glass to protect against explosion
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Don't use cracked or broken containers
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Clean up any broken glass immediately and dispose of it properly
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Carry chemical containers in bucket or other container; use a tray, rack, or cart rather than carrying by hand
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Have a partner present for jobs with highly hazardous substances
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Cover work surfaces with removable absorbent plastic-backed paper when working with highly hazardous substances
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Dispose of chemical waste in properly labeled receptacles
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Never pour chemical waste down drains or into general trash
Practice Good Hygiene
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Don't eat, drink, smoke, chew gum, or apply cosmetics in areas with lab chemicals
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Always wash hands carefully before entering and leaving lab
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Don't keep food, beverages, or utensils in chemical storage areas (including refrigerators)
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Don't drink from lab glasses
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Don't smell or taste chemicals
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Don't use mouth suction to pipette or start a siphon
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Keep work area neat
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Never store or place materials in hallways, on floor, or blocking exits or emergency equipment
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If an experiment must be left unattended, leave lights on and a warning sign on the door
Prevent Fires, Shock, and Other Lab Hazards
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Be sure wires and plugs aren't frayed or broken
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Use tongs or heat-resistant gloves with autoclaves and equipment that uses heat
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Don't touch electrical equipment with wet hands or while standing on wet floor
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Keep clothing/long hair away from equipment with moving parts
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Keep compressed gas cylinders clean, free of oil and grease, and chained in place
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Only operate equipment you've been trained to use
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Immediately report any equipment that's not working properly
Act Promptly and Properly in an Emergency
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Clean up small spills immediately; contact emergency response team if appropriate
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Know how to turn on fire alarm, who to contact, and evacuation route to take
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For chemical inhalation, get to fresh air and seek medical attention
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For chemical eye contact, immediately flush eyes with water for at least 15 minutes and seek medical attention
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For chemical ingestion, check substance's MSDS for instructions and call poison control
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For chemical skin contact, wash immediately and thoroughly with water; remove and dispose of contaminated clothing properly; seek medical attention if symptoms persist
Use Common Sense
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Be alert to any possibly unsafe situations and report immediately
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Never indulge in horseplay in the lab or chemical storage area
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If you see something, say something